Molecular Biology Techniques: A Classroom Laboratory Manual, 5th Edition

Molecular Biology Techniques: A Classroom Laboratory Manual, Fifth Edition, is an in-depth laboratory manual for upper-level undergraduates and beginning graduate students in the general field of molecular biology techniques and biochemical applications. Presented as a series of coordinated experiments that mimics either a one- or two-semester research experience, the book also provides theories and explanations behind the techniques. Topics include manipulation of recombinant DNA, protein expression and purification, analysis of mRNA levels, mammalian cell culture techniques, CRISPR-mediated gene knockdown, and more. New topics, such as Gibson assembly experiments, have also been included. For instructors, the book contains easy to follow guides and preparation lists, designed to facilitate lab setup and pre-lab questions for use as a teaching tool.

Χαρακτηριστικά:

• Presents student-tested labs proven successful in real classroom laboratories.
• Covers updated lab sessions that focus on in silico methods.
• Includes discussion questions and further online resources for students, as well as lab preparation lists for instructor use.
• Also includes updates such as Gibson assembly experiments and lab session flow charts for easier selection.

Περιεχόμενα:

1. DNA MANIPULATION Lab session 1-5
2. SCREENING TRANSFORMANTS Lab session 6-8
3. EXPRESSION, PURIFICATION, AND ANALYSIS OF RECOMBINANT PROTEINS FROM BACTERIA Lab session 9-12b
4. ANALYSIS OF mRNA LEVELS Lab Session 13-17
5. MODULATION OF GENE EXPRESSION Lab Session 18-30
6. IN SILICO TOOLS Lab Session 31-33